Cytokeratin 79 Rekombinanter Antikörper

Cytokeratin 79 Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

250927D8

Kat-Nr. : 86310-1-RR

Synonyme

CK-79, Cytokeratin-79, K6L, K79, KB38

---

• 

  WB analysis of mouse skin using 86310-1-RR

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 86310-1-RR (KRT79 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 86310-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86310-1-RR (Cytokeratin 79 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 86310-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 86310-1-RR (Cytokeratin 79 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 86310-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86310-1-RR (Cytokeratin 79 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skin using 86310-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 79 antibody (86310-1-RR, Clone: 250927D8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushautgewebe
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, Maushautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushautgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86310-1-RR bindet in WB, IHC, IF-P, ELISA Cytokeratin 79 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: keratin 79
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC039148
• Gene symbol: KRT79
• Gene ID (NCBI): 338785
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KRT79(Keratin 79, also known as K6L or KRT6L) is a type II epithelial keratin, which is mainly expressed in skeletal muscle, skin, scalp and other tissues and participates in maintaining the structural integrity of epithelial cells. KRT79 plays an important role in hair follicle development, and it is one of the marker genes of hair follicle outer root sheath and hair stromal cells (HG cells). Together with KRT17 and SOX9, it marks HG cells and participates in hair follicle regeneration and morphogenesis.