Cytokeratin 8 Monoklonaler Antikörper

Cytokeratin 8 Monoklonal Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F2H5

Kat-Nr. : 68627-1-PBS

Synonyme

KRT8, Cytokeratin,Keratin, CK-8, CK8, CK 8

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  WB analysis using 68627-1-Ig (same clone as 68627-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 68627-1-Ig (same clone as 68627-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

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  WB analysis of rat colon using 68627-1-Ig (same clone as 68627-1-PBS)

  rat colon tissue were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 68627-1-Ig (same clone as 68627-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 68627-1-Ig (same clone as 68627-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 68627-1-Ig (same clone as 68627-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 68627-1-Ig (same clone as 68627-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 68627-1-Ig (same clone as 68627-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51080-1

  Cytometric bead array standard curve of MP51080-1, Cytokeratin 8 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68627-1-PBS. Detection antibody: 68627-2-PBS. Standard:Ag0488. Range: 0.098-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

68627-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA Cytokeratin 8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 8 fusion protein Ag0488
• Vollständiger Name: keratin 8
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 50-52 kDa
• GenBank-Zugangsnummer: BC000654
• Gene symbol: Cytokeratin 8
• Gene ID (NCBI): 3856
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.