Cytokeratin 8 Rekombinanter Antikörper

Cytokeratin 8 Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251045E11

Kat-Nr. : 86457-4-PBS

Synonyme

KRT8, CARD2, CK 8, CK8, CK-8

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  WB analysis using 86457-4-RR (same clone as 86457-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86457-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsil using 86457-4-RR (same clone as 86457-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86457-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsil using 86457-4-RR (same clone as 86457-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86457-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 86457-4-RR (same clone as 86457-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86457-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 86457-4-RR (same clone as 86457-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86457-4-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 86457-4-RR (same clone as 86457-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86457-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 86457-4-RR (same clone as 86457-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86457-4-PBS in a different storage buffer formulation.

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  IHC staining of Human Kidney(renal cell) Cancer using 86457-4-RR (same clone as 86457-4-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86457-4-PBS in a different storage buffer formulation.

• 

  IHC staining of Human Kidney(renal cell) Cancer using 86457-4-RR (same clone as 86457-4-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86457-4-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 86457-4-RR (same clone as 86457-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86457-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 86457-4-RR (same clone as 86457-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86457-4-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 86457-4-RR (same clone as 86457-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 8 antibody (86457-4-RR, Clone: 251045E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86457-4-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 86457-4-RR (same clone as 86457-4-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 8 antibody (86457-4-RR, Clone: 251045E11 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86457-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86457-4-RR (same clone as 86457-4-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 8 antibody (86457-4-RR, Clone: 251045E11 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86457-4-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

86457-4-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA Cytokeratin 8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: keratin 8
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC000654
• Gene symbol: Cytokeratin 8
• Gene ID (NCBI): 3856
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytokeratin 8 (CK8) is a protein that belongs to the cytokeratin family, which is a group of structural proteins found in the intracytoplasmic cytoskeleton of epithelial cells. CK8 forms intermediate filaments within the cytoplasm of simple epithelial cells, typically as a dimer with cytokeratin 18 (CK18). These filaments provide a stabilizing framework that helps determine cell shape and enables cells to cope with mechanical stress. CK8/CK18 filaments also serve as a mesh of "paths" for signaling molecules, metabolites, and pathogens to travel within the cell in an organized manner. CK8 is expressed in various epithelial tissues and is particularly associated with glandular and simple epithelia. Its expression is also a significant marker in several types of cancer, including lung and esophageal cancer, where it can indicate tumor progression and poor prognosis.