DAAM1 Monoklonaler Antikörper

DAAM1 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3F11F11

Kat-Nr. : 67287-1-Ig

Synonyme

3F11F11, Disheveled-associated activator of morphogenesis 1, FLJ41657, KIAA0666

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  WB analysis using 67287-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67287-1-Ig (DAAM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 67287-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 67287-1-Ig (DAAM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 67287-1-Ig

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67287-1-Ig (DAAM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 67287-1-Ig

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67287-1-Ig (DAAM1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67287-1-Ig bindet in WB, IHC, ELISA DAAM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DAAM1 fusion protein Ag6809
• Vollständiger Name: dishevelled associated activator of morphogenesis 1
• Berechnetes Molekulargewicht: 123 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC064999
• Gene symbol: DAAM1
• Gene ID (NCBI): 23002
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dishevelled-associated activator of morphogenesis 1 (Daam1) is a member of the formin protein family that is implicated in actin assembly. Daam1 also plays a critical role in mediating Wnt/Fz signaling pathway and regulates cell polarity during development and tissue homoeostasis. This antibody well recognizes the endogenous DAAM1 protein around 120 kDa.