DAAM2 Polyklonaler Antikörper

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• KD/KO Validated

DAAM2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25206-1-PBS

Synonyme

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  WB analysis of mouse brain using 25206-1-AP (same clone as 25206-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25206-1-AP (DAAM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25206-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 25206-1-AP (same clone as 25206-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25206-1-AP (DAAM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25206-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Transfected HEK-293 using 25206-1-AP (same clone as 25206-1-PBS)

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 25206-1-AP (DAAM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25206-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 25206-1-AP (same clone as 25206-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25206-1-AP (DAAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25206-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 25206-1-AP (same clone as 25206-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25206-1-AP (DAAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25206-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 25206-1-AP (same clone as 25206-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25206-1-AP (DAAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25206-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 25206-1-AP (same clone as 25206-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25206-1-AP (DAAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25206-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 25206-1-AP (same clone as 25206-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DAAM2 antibody (25206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25206-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25206-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA DAAM2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DAAM2 fusion protein Ag18955
• Vollständiger Name: dishevelled associated activator of morphogenesis 2
• Berechnetes Molekulargewicht: 1068 aa, 123 kDa
• Beobachtetes Molekulargewicht: 100-130 kDa
• GenBank-Zugangsnummer: BC128388
• Gene symbol: DAAM2
• Gene ID (NCBI): 23500
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.