DAB1 Polyklonaler Antikörper

DAB1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31459-1-AP

Synonyme

Disabled homolog 1

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  WB analysis using 31459-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31459-1-AP (DAB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 31459-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31459-1-AP (DAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 31459-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 31459-1-AP (DAB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 31459-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31459-1-AP (DAB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 31459-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31459-1-AP (DAB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 31459-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31459-1-AP (DAB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 31459-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DAB1 antibody (31459-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Cerebellum-Gewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31459-1-AP bindet in WB, IHC, IF/ICC, ELISA DAB1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DAB1 fusion protein Ag35376
• Vollständiger Name: disabled homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 45 kDa, 80 kDa
• GenBank-Zugangsnummer: NM_021080
• Gene symbol: DAB1
• Gene ID (NCBI): 1600
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Disabled-1 (Dab1) is an adaptor protein that is essential for neuronal migration and maturation in response to the extracellular protein Reelin. DAB1 protein docks to the intracellular part of the Reelin very low density lipoprotein receptor and apoE receptor type 2 and becomes tyrosine-phosphorylated following binding of Reelin to cortical neurons. The DAB1 protein contains a 180-amino acid N-terminal protein interaction/phosphotyrosine-binding (PTB) 1 domain that docks to the short cytoplasmic tail of the very low density lipoprotein receptor or apoE receptor type 2 at the level of NPXY motifs, with a preference for unphosphorylated motifs. Dab1 polypeptide bands ranging from 36 to 120 kDa have been identified in mouse embryonic brain, the 80-kDa Dab1 being the predominant form (PMID: 24313315).