DAB1 Polyklonaler Antikörper

DAB1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31459-1-PBS

Synonyme

Disabled homolog 1

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  WB analysis using 31459-1-AP (same clone as 31459-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31459-1-AP (DAB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 31459-1-AP (same clone as 31459-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31459-1-AP (DAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 31459-1-AP (same clone as 31459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 31459-1-AP (DAB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 31459-1-AP (same clone as 31459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31459-1-AP (DAB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 31459-1-AP (same clone as 31459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31459-1-AP (DAB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 31459-1-AP (same clone as 31459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31459-1-AP (DAB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 31459-1-AP (same clone as 31459-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DAB1 antibody (31459-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

31459-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA DAB1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DAB1 fusion protein Ag35376
• Vollständiger Name: disabled homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 45 kDa, 80 kDa
• GenBank-Zugangsnummer: NM_021080
• Gene symbol: DAB1
• Gene ID (NCBI): 1600
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Disabled-1 (Dab1) is an adaptor protein that is essential for neuronal migration and maturation in response to the extracellular protein Reelin. DAB1 protein docks to the intracellular part of the Reelin very low density lipoprotein receptor and apoE receptor type 2 and becomes tyrosine-phosphorylated following binding of Reelin to cortical neurons. The DAB1 protein contains a 180-amino acid N-terminal protein interaction/phosphotyrosine-binding (PTB) 1 domain that docks to the short cytoplasmic tail of the very low density lipoprotein receptor or apoE receptor type 2 at the level of NPXY motifs, with a preference for unphosphorylated motifs. Dab1 polypeptide bands ranging from 36 to 120 kDa have been identified in mouse embryonic brain, the 80-kDa Dab1 being the predominant form (PMID: 24313315).