DAB2 Rekombinanter Antikörper

DAB2 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250409B4

Kat-Nr. : 86141-1-PBS

Synonyme

Adaptor molecule disabled-2, Differentially expressed in ovarian carcinoma 2, Differentially-expressed protein 2, Disabled homolog 2, DOC 2

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  WB analysis using 86141-1-RR (same clone as 86141-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86141-1-RR (DAB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86141-1-PBS in a different storage buffer formulation.

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  WB analysis of Caco-2 using 86141-1-RR (same clone as 86141-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 86141-1-RR (DAB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86141-1-PBS in a different storage buffer formulation.

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  Sample test of MP02525-1

  The mean DAB2 concentration was determined to be 34.8 ng/mL in Hela cell extract based on a 1.1 mg/mL extract load.

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  IF Staining of RAW 264.7 using 86141-1-RR (same clone as 86141-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed RAW 264.7 cells using DAB2 antibody (86141-1-RR, Clone: 250409B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86141-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02525-1

  Cytometric bead array standard curve of MP02525-1, DAB2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86141-1-PBS. Detection antibody: 86141-3-PBS. Standard: Ag0162. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 86141-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86141-1-RR against Human DAB2 were performed. The affinity constant is 3.07 nM.

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Geprüfte Anwendungen

Produktinformation

86141-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test DAB2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DAB2 fusion protein Ag0162
• Vollständiger Name: disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila)
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 96 kDa
• GenBank-Zugangsnummer: BC003064
• Gene symbol: DAB2
• Gene ID (NCBI): 1601
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DAB2 is a protein of 770 amino acid residues with a predicted molecular weight of 85.5kDa. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2-like domains, alpha and beta). Human DAB2 has an overall 83% identify with the mouse p96 protein, a putative mitogen-responsive phosphoprotein; homology is strongest in the amino-terminal end of the protein in a region corresponding to the phosphotyrosine interaction domain (PID), and contains multiple SH3 binding motifs. Chromosomal localization by FISH showed that the DAB2 gene is located on 5p13. The expression of DAB2 is down-regulated or absent in all the carcinoma cell lines examined, including prostate and ovarian carcinoma cell lines. The N-terminal domain of DAB2 interacts with Dishevelled-3 (Dvl-3), a signaling mediator of the Wnt pathway. Ectopic expression of DAB2 attenuates canonical Wnt/catenin-mediated signaling, including accumulation of catenin and cyclin D1 induction. DAB2 suppresses both protein kinase C and peptide growth factor-elicited signal pathways via the Ras-mitogen-activated protein kinase pathway. The proline-rich domain of DAB2 also interacts with proteins containing SH3 domain, such as Src and Fgr. The binding of DAB2 to c-Src resultes in the inactivation of c-Src. All data suggest that DAB2 is a potent tumor suppressor in many cancer types.