DAB2 Rekombinanter Antikörper

DAB2 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250409B4

Kat-Nr. : 86141-1-RR

Synonyme

Adaptor molecule disabled-2, Differentially expressed in ovarian carcinoma 2, Differentially-expressed protein 2, Disabled homolog 2, DOC 2

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  WB analysis using 86141-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86141-1-RR (DAB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 86141-1-RR

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 86141-1-RR (DAB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 86141-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed RAW 264.7 cells using DAB2 antibody (86141-1-RR, Clone: 250409B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86141-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86141-1-RR against Human DAB2 were performed. The affinity constant is 3.07 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Caco-2-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IF/ICC: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86141-1-RR bindet in WB, IF/ICC, ELISA DAB2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DAB2 fusion protein Ag0162
• Vollständiger Name: disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila)
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 96 kDa
• GenBank-Zugangsnummer: BC003064
• Gene symbol: DAB2
• Gene ID (NCBI): 1601
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DAB2 is a protein of 770 amino acid residues with a predicted molecular weight of 85.5kDa. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2-like domains, alpha and beta). Human DAB2 has an overall 83% identify with the mouse p96 protein, a putative mitogen-responsive phosphoprotein; homology is strongest in the amino-terminal end of the protein in a region corresponding to the phosphotyrosine interaction domain (PID), and contains multiple SH3 binding motifs. Chromosomal localization by FISH showed that the DAB2 gene is located on 5p13. The expression of DAB2 is down-regulated or absent in all the carcinoma cell lines examined, including prostate and ovarian carcinoma cell lines. The N-terminal domain of DAB2 interacts with Dishevelled-3 (Dvl-3), a signaling mediator of the Wnt pathway. Ectopic expression of DAB2 attenuates canonical Wnt/catenin-mediated signaling, including accumulation of catenin and cyclin D1 induction. DAB2 suppresses both protein kinase C and peptide growth factor-elicited signal pathways via the Ras-mitogen-activated protein kinase pathway. The proline-rich domain of DAB2 also interacts with proteins containing SH3 domain, such as Src and Fgr. The binding of DAB2 to c-Src resultes in the inactivation of c-Src. All data suggest that DAB2 is a potent tumor suppressor in many cancer types.