DAB2IP Polyklonaler Antikörper

DAB2IP Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30020-1-AP

Synonyme

DAB2 interacting protein, ASK-interacting protein 1, ASK interacting protein 1, AIP-1, AIP1

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  WB analysis using 30020-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30020-1-AP (DAB2IP antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30020-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30020-1-AP (DAB2IP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of 4T1 using 30020-1-AP

  IP result of anti-DAB2IP (IP:30020-1-AP, 4ug; Detection:30020-1-AP 1:6000) with 4T1 cells lysate 2800 ug.

• 

  IP experiment of 4T1 using 30020-1-AP

  IP result of anti-DAB2IP (IP:30020-1-AP, 4ug; Detection:30020-1-AP 1:6000) with 4T1 cells lysate 4800 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HepG2-Zellen, Rattenhirngewebe
• Erfolgreiche IP: 4T1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30020-1-AP bindet in WB, IP, ELISA DAB2IP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DAB2IP fusion protein Ag31340
• Vollständiger Name: DAB2 interacting protein
• Berechnetes Molekulargewicht: 1189 aa, 132 kDa
• Beobachtetes Molekulargewicht: 125-132 kDa
• GenBank-Zugangsnummer: BC146762
• Gene symbol: DAB2IP
• Gene ID (NCBI): 153090
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Disabled homolog 2-interacting protein (DAB2IP) is also named as ASK-interacting protein 1 (AIP-1). DAB2IP, is a new member of the Ras GTPase-activating protein family. It interacts directly with DAB2 and has distinct cellular functions such as modulating different signal cascades associated with cell proliferation, survival, apoptosis and metastasis. The aberrant alteration of DAB2IP in cancer is caused by a variety of mechanisms, including the aberrant promoter methylation, histone deacetylation, and others (PMID: 26658103). DAB2IP is reported to bind with Apoptosis signal-regulating kinase 1 (ASK1), and is called ASK1 Interacting Protein (AIP1) (PMID: 12813029). DAB2IP function is that of a tumor suppressor in tumor cell growth, metastasis, and other aspects in cancer progression. In prostate cancer, DAB2IP expression was repressed by promoter methylation and histone modification (PMID: 12446720, PMID: 15817459).