DACH1 Polyklonaler Antikörper

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• KD/KO Validated

DACH1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(76)

Rezensionen (1)

Kat-Nr. : 10914-1-AP

Synonyme

DACH, Dachshund-1

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  WB analysis using 10914-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10914-1-AP (DACH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10914-1-AP

  WB result of DACH1 (10914-1-AP, 1:4000) with si-control and si-DACH1 transfected HEK293 cells.

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  IP experiment of HEK-293 using 10914-1-AP

  IP result of anti-DACH1 (IP:10914-1-AP, 4ug; Detection:10914-1-AP 1:1000) with HEK-293 cells lysate 6000ug.

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  IHC staining of human oesophagus cancer using 10914-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10914-1-AP (DACH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 10914-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10914-1-AP (DACH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10914-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10914-1-AP (DACH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10914-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10914-1-AP (DACH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 10914-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DACH1 antibody (10914-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: H9C2-Zellen, HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophaguskarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 28 publications below
• IHC: See 31 publications below
• IF: See 40 publications below
• IP: See 1 publications below
• ChIP: See 2 publications below

Produktinformation

10914-1-AP bindet in WB, IHC, IF/ICC, IP, ChIP, ELISA DACH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DACH1 fusion protein Ag1354
• Vollständiger Name: dachshund homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 79 kDa
• Beobachtetes Molekulargewicht: 97-110 kDa
• GenBank-Zugangsnummer: BC021219
• Gene symbol: DACH1
• Gene ID (NCBI): 1602
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells. Dach1 expression is enriched in rECs, which are associated with osteoprogenitors and bone-resorbing osteoclasts, and overexpression of DACH1 in postnatal mice induces a strong increase in arteries and type R capillaries, leading to local metabolic changes and enabling trabecular bone formation in normally highly hypoxic areas of the diaphysis (PMID: 39528700). Moreover, Loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human DACH1.

Publikationen

Species	Application	Title
mouse	IF	Nature; Single-cell analysis of early progenitor cells that build coronary arteries.; Authors - Tianying Su
human	IF	Nature; Excitation-induced ataxin-3 aggregation in neurons from patients with Machado-Joseph disease.; Authors - Koch Philipp P
mouse	IF	Science; Breathing control center neurons that promote arousal in mice.; Authors - Kevin Yackle
human	IF	Cell Stem Cell; Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders.; Authors - Carmen Lorenz
mouse	IHC	Nat Cell Biol; A single-cell transcriptional roadmap for cardiopharyngeal fate diversification.; Authors - Wei Wang
human	WB	Nat Neurosci; Auto-attraction of neural precursors and their neuronal progeny impairs neuronal migration.; Authors - Julia Ladewig

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (11-02-2021)

This antibody works great for IF. It specifically marked DACH1-positive coronary vessel cells in mouse heart.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Heart