DACH1 Monoklonaler Antikörper

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• KD/KO Validated

DACH1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

3B6D2

Kat-Nr. : 60082-1-Ig

Synonyme

DACH, DACH1, Dachshund homolog 1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 60082-1-Ig

  WB result of DACH1 (60082-1-Ig, 1:5000) with si-control and si-DACH1 transfected HEK293 cells.

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  WB analysis of MCF-7 using 60082-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 60082-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 60082-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 60082-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 60082-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60082-1-Ig (DACH1 antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 60082-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60082-1-Ig (DACH1 antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 60082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 60082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60082-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 60082-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse brain using 60082-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 60082-1-Ig (DACH1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse embryo using 60082-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse embryo using 60082-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, C6-Zellen, HEK-293-Zellen, L02-Zellen, ROS1728-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Endometriumkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen, Mausembryogewebe, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:10-1:100
• Immunfluoreszenz (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

60082-1-Ig bindet in WB, IHC, IF, ELISA DACH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DACH1 fusion protein Ag4474
• Vollständiger Name: dachshund homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 79 kDa
• Beobachtetes Molekulargewicht: 97-110 kDa
• GenBank-Zugangsnummer: BC021219
• Gene symbol: DACH1
• Gene ID (NCBI): 1602
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing, but isoform1(97-110kd) is the predominantly expressed form in tissue. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human DACH1.

Publikationen

Species	Application	Title
human	WB	Cell Biol Int; DACH1 inhibits the proliferation and migration of papillary thyroid carcinoma; Authors - Shengru Liang
human	WB	Front Oncol; Identification and verification of the temozolomide resistance feature gene DACH1 in gliomas; Authors - Qiang Gu ; KD Validated