DACH1 Monoklonaler Antikörper

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• KD/KO Validated

DACH1 Monoklonal Antikörper für IF-P

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B6D2

Kat-Nr. : CL488-60082

Synonyme

Dachshund-1, DACH, 3B6D2

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  IF Staining of mouse brain using CL488-60082

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-60082 (DACH1 antibody) at dilution of 1:50.

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  IF Staining of mouse brain using CL488-60082

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-60082 (DACH1 antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-60082 bindet in IF-P DACH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DACH1 fusion protein Ag4474
• Vollständiger Name: dachshund homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 79 kDa
• GenBank-Zugangsnummer: BC021219
• Gene symbol: DACH1
• Gene ID (NCBI): 1602
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells. Dach1 expression is enriched in rECs, which are associated with osteoprogenitors and bone-resorbing osteoclasts, and overexpression of DACH1 in postnatal mice induces a strong increase in arteries and type R capillaries, leading to local metabolic changes and enabling trabecular bone formation in normally highly hypoxic areas of the diaphysis (PMID: 39528700). Moreover, Loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing, but isoform1(97-110kd) is the predominantly expressed form in tissue. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human DACH1. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.