DAK Polyklonaler Antikörper

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• KD/KO Validated

DAK Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 12224-1-AP

Synonyme

FAD-AMP lyase, DHA kinase, cyclizing, cyclic FMN forming, Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase

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  WB analysis of HeLa using 12224-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12224-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12224-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12224-1-AP

  WB result of DAK antibody (12224-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-DAK transfected HeLa cells.

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  WB analysis of mouse uterus using 12224-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12224-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12224-1-AP

  IP result of anti-DAK (IP:12224-1-AP, 3ug; Detection:12224-1-AP 1:500) with HeLa cells lysate 2150ug.

• 

  IHC staining of human stomach using 12224-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12224-1-AP (DAK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Maus-Uterusgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

12224-1-AP bindet in WB, IHC, IF, IP, ELISA DAK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DAK fusion protein Ag2861
• Vollständiger Name: dihydroxyacetone kinase 2 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 575 aa, 59 kDa
• Beobachtetes Molekulargewicht: 55-59 kDa
• GenBank-Zugangsnummer: BC001341
• Gene symbol: DAK
• Gene ID (NCBI): 26007
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DAK, also name as TKFC, Triokinase, and FMN cyclase, catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. DAK has some isoforms with MW 51-55 kDa and 59 kDa.

Publikationen

Species	Application	Title
mouse	WB	Endocrinology; Hepatic but not intestinal FBP1 is required for fructose metabolism and tolerance; Authors - Qiufang Bai
human	WB,IF	iScience; CircSOBP suppresses the progression of glioma by disrupting glycolysis and promoting the MDA5-mediated immune response; Authors - Maolin Mu ; KO Validated
rat	WB	Mol Psychiatry; Proteasome-independent K63 polyubiquitination selectively regulates ATP levels and proteasome activity during fear memory formation in the female amygdala; Authors - Kayla Farrell
human	WB	Pharmaceuticals (Basel); Magnolol Inhibits High Fructose-Induced Podocyte Inflammation via Downregulation of TKFC/Sp1/HDAC4/Notch1 Activation; Authors - Ziang Zhou