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DAPK2-Specific Polyklonaler Antikörper

DAPK2-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20048-1-PBS

Synonyme

DAPK2, DAP kinase 2, DAP kinase related protein 1, DAP-kinase-related protein 1, Death-associated protein kinase 2

Filter:
  • Western Blot (WB) analysis of various lysates using DAPK2-Specific Polyclonal antibody (20048-1-AP)

    WB analysis using 20048-1-AP (same clone as 20048-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DAPK2-Specific Polyclonal antibody (20048-1-AP)

    WB analysis of HeLa using 20048-1-AP (same clone as 20048-1-PBS)

    WB result of DAPK2-Specific antibody (20048-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DAPK2-Specific transfected HeLa cells. This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using DAPK2-Specific Polyclonal antibody (20048-1-AP)

    WB analysis of A431 using 20048-1-AP (same clone as 20048-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using DAPK2-Specific Polyclonal antibody (20048-1-AP)

    WB analysis of A431 using 20048-1-AP (same clone as 20048-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using DAPK2-Specific Polyclonal antibody (20048-1-AP)

    IHC staining of mouse skeletal muscle using 20048-1-AP (same clone as 20048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using DAPK2-Specific Polyclonal antibody (20048-1-AP)

    IHC staining of mouse skeletal muscle using 20048-1-AP (same clone as 20048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using DAPK2-Specific Polyclonal antibody (20048-1-AP)

    IF Staining of A375 using 20048-1-AP (same clone as 20048-1-PBS)

    Immunofluorescent analysis of A375 cells using 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

20048-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA DAPK2-Specific und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name death-associated protein kinase 2
Berechnetes Molekulargewicht 43 kDa
Beobachtetes Molekulargewicht 38-43 kDa
GenBank-ZugangsnummerNM_014326
Gene symbol DAPK2
Gene ID (NCBI) 23604
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DAPK2, also named as DRP-1, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. DAPK2 is a calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK2 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to DAPK2.