DAPK3 Polyklonaler Antikörper

DAPK3 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 30656-1-AP

Synonyme

ZIP kinase, EC:2.7.11.1, Dlk, Death-associated protein kinase 3, DAP-like kinase

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  WB analysis using 30656-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30656-1-AP (DAPK3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 30656-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DAPK3 antibody (30656-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

30656-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA DAPK3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DAPK3 fusion protein Ag33051
• Vollständiger Name: death-associated protein kinase 3
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 37, 52 kDa
• GenBank-Zugangsnummer: NM_001348
• Gene symbol: DAPK3
• Gene ID (NCBI): 1613
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DAPK3, also named as ZIPK and Dlk, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. The calculated molecular weight of DAPK3 is 52 kDa. This antibody can recognize the 37 kDa isoform of target. ZIP kinase is a Serine/threonine kinase which acts as a positive regulator of apoptosis. ZIP kinasephosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. ZIP kinase regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. It catalyze the reaction: ATP + a protein = ADP + a phosphoprotein.

Publikationen

Species	Application	Title
mouse	WB,CoIP	Biochem Biophys Res Commun; Protection of blood-brain barrier by endothelial DAPK1 deletion after stroke; Authors - Zhijiang Gu
human,rat	WB,IHC,IF,CoIP	Acta Biochim Biophys Sin (Shanghai); ZIPK collaborates with STAT5A in p53-mediated ROS accumulation in hyperglycemia-induced vascular injury; Authors - Qichao Wu ; KD Validated