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DAPK3 Polyklonaler Antikörper

DAPK3 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30656-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using DAPK3 Polyclonal antibody (30656-1-AP)

    WB analysis using 30656-1-AP (same clone as 30656-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30656-1-AP (DAPK3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30656-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DAPK3 Polyclonal antibody (30656-1-AP)

    IF Staining of A431 using 30656-1-AP (same clone as 30656-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DAPK3 antibody (30656-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30656-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30656-1-PBS bindet in WB, IF/ICC, Indirect ELISA DAPK3 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen DAPK3 fusion protein Ag33051
Vollständiger Name death-associated protein kinase 3
Berechnetes Molekulargewicht 53 kDa
Beobachtetes Molekulargewicht37, 52 kDa
GenBank-ZugangsnummerNM_001348
Gene symbol DAPK3
Gene ID (NCBI) 1613
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DAPK3, also named as ZIPK and Dlk, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. The calculated molecular weight of DAPK3 is 52 kDa. This antibody can recognize the 37 kDa isoform of target. ZIP kinase is a Serine/threonine kinase which acts as a positive regulator of apoptosis. ZIP kinasephosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. ZIP kinase regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. It catalyze the reaction: ATP + a protein = ADP + a phosphoprotein.