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DAPP1 Rekombinanter Antikörper

DAPP1 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242582D1

Kat-Nr. : 85346-1-PBS

Synonyme

BAM32, B-cell adapter molecule of 32 kDa, DAPP 1, Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide, hDAPP1

Filter:
  • Western Blot (WB) analysis of various lysates using DAPP1 Recombinant antibody (85346-1-RR)

    WB analysis using 85346-1-RR (same clone as 85346-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85346-1-RR (DAPP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. Jurkat cells as negative control. This data was developed using the same antibody clone with 85346-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85346-1-RR

    Affinity and Kinetic Characterization of 85346-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85346-1-RR against Human DAPP1 were performed. The affinity constant is 8.12 nM.

Geprüfte Anwendungen

Produktinformation

85346-1-PBS bindet in WB, Indirect ELISA DAPP1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen DAPP1 fusion protein Ag6459
Vollständiger Name dual adaptor of phosphotyrosine and 3-phosphoinositides
Berechnetes Molekulargewicht 32 kDa
Beobachtetes Molekulargewicht32 kDa
GenBank-ZugangsnummerBC012924
Gene symbol DAPP1
Gene ID (NCBI) 27071
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DAPP1 was identified as dual adaptor for phosphotyrosine and 3-phosphoinositides, a novel 280 amino acid protein which contains a putative myristoylation site at its N-terminus followed by a SH2 and a PH domain at its C-terminus (PMID: 10432293). DAPP1 is also referred to B cell adaptor molecule of 32 kDa (Bam32), and is expressed by B lymphocytes, but not T lymphocytes or nonhematopoietic cells. DAPP1/Bam32 was shown to regulate BCR signaling downstream of phosphatidylinositol 3-kinase (PI3K) (PMID: 10770799). Furthermore, DAPP1/Bam32 may server to integrate PI3K and Src kinase signaling to promote Rac-dependent B cell adhesive interactions important for antigen presentation function (PMID: 20495066).