DARS2 Rekombinanter Antikörper

DARS2 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

243050E5

Kat-Nr. : 85691-2-PBS

Synonyme

Aspartate  tRNA ligase, Aspartate--tRNA ligase, mitochondrial, Aspartyl-tRNA synthetase, AspRS, EC:6.1.1.12

---

• 

  WB analysis using 85691-2-RR (same clone as 85691-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85691-2-RR (DARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85691-2-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 85691-2-RR (same clone as 85691-2-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 85691-2-RR (DARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85691-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 85691-2-RR (same clone as 85691-2-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 85691-2-RR (DARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85691-2-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 85691-2-RR (same clone as 85691-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DARS2 antibody (85691-2-RR, Clone: 243050E5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85691-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP02026-2

  Cytometric bead array standard curve of MP02026-2, DARS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85691-3-PBS. Detection antibody: 85691-2-PBS. Standard: Ag4809. Range: 0.781-100 ng/mL.

---

Geprüfte Anwendungen

Produktinformation

85691-2-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA DARS2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DARS2 fusion protein Ag4809
• Vollständiger Name: aspartyl-tRNA synthetase 2, mitochondrial
• Berechnetes Molekulargewicht: 645 aa, 74 kDa
• Beobachtetes Molekulargewicht: 66-74 kDa
• GenBank-Zugangsnummer: BC045173
• Gene symbol: DARS2
• Gene ID (NCBI): 55157
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DARS2 is also named as AspRS (aspartyl-tRNA synthetase) and belongs to the class-II aminoacyl-tRNA synthetase family. The deduced 645-amino acid protein has a 47-amino acid mitochondrial targeting signal, resulting in a mature protein of 598 amino acids. DARS2 contains conserved residues involved in ATP binding, tRNA binding, and aspartic acid recognition, as well as catalytic site motifs characteristic of amino acid tRNA synthetases. It is a dimeric proteins(PMID:19443655). Rat aspartyl-tRNA synthetase has a N-terminal polypeptide extension of about 40 amino acid residues which can be removed without impairing its catalytic activity(PMID:9030747). This protein has different molecular mass(55 kDa, 50 kDa,66 kDa ) according to the publications(PMID:11306575; 19443655;15299749).