DARS2 Rekombinanter Antikörper

DARS2 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

243050E5

Kat-Nr. : 85691-2-RR

Synonyme

Aspartate  tRNA ligase, Aspartate--tRNA ligase, mitochondrial, Aspartyl-tRNA synthetase, AspRS, EC:6.1.1.12

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  WB analysis using 85691-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85691-2-RR (DARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 85691-2-RR

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 85691-2-RR (DARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 85691-2-RR

  Jurkat cells were subjected to SDS PAGE followed by western blot with 85691-2-RR (DARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 85691-2-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DARS2 antibody (85691-2-RR, Clone: 243050E5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, A549-Zellen, HepG2-Zellen, Jurkat-Zellen, LNCaP-Zellen, NCI-H1299-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85691-2-RR bindet in WB, IF/ICC, ELISA DARS2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DARS2 fusion protein Ag4809
• Vollständiger Name: aspartyl-tRNA synthetase 2, mitochondrial
• Berechnetes Molekulargewicht: 645 aa, 74 kDa
• Beobachtetes Molekulargewicht: 66-74 kDa
• GenBank-Zugangsnummer: BC045173
• Gene symbol: DARS2
• Gene ID (NCBI): 55157
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DARS2 is also named as AspRS (aspartyl-tRNA synthetase) and belongs to the class-II aminoacyl-tRNA synthetase family. The deduced 645-amino acid protein has a 47-amino acid mitochondrial targeting signal, resulting in a mature protein of 598 amino acids. DARS2 contains conserved residues involved in ATP binding, tRNA binding, and aspartic acid recognition, as well as catalytic site motifs characteristic of amino acid tRNA synthetases. It is a dimeric proteins(PMID:19443655). Rat aspartyl-tRNA synthetase has a N-terminal polypeptide extension of about 40 amino acid residues which can be removed without impairing its catalytic activity(PMID:9030747). This protein has different molecular mass(55 kDa, 50 kDa,66 kDa ) according to the publications(PMID:11306575; 19443655;15299749).