Dopamine Transporter/DAT Polyklonaler Antikörper

Dopamine Transporter/DAT Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22524-1-PBS

Synonyme

2, SLC6A3, DA transporter, DAT, DAT1

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  WB analysis using 22524-1-AP (same clone as 22524-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22524-1-AP (DAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

  IP result of anti-Dopamine Transporter/DAT (IP:22524-1-AP, 4ug; Detection:22524-1-AP 1:1000) with mouse brain tissue lysate 1720 ug. This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DAT antibody (22524-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using Dopamine Transporter/DAT antibody (22524-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22524-1-PBS bindet in WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA Dopamine Transporter/DAT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Dopamine Transporter/DAT fusion protein Ag18297
• Vollständiger Name: solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
• Berechnetes Molekulargewicht: 620 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC133003
• Gene symbol: DAT
• Gene ID (NCBI): 6531
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DAT, also name as SLC6A3, is dopamine transporter which is a member of the sodium- and chloride- dependent neurotransmitter transporter family. Dopamine(DA) released from neurons is cleared by the DA transporter (DAT). Altered dopaminergic signaling is linked to multiple neuropsychiatric disorders, such as attention deficit hyperactive disorder, mood disorders, schizophrenia, autism and so on (PMID:30755521). 22524-1-AP can detect 70~80kDa band, which is consistent with the report (PMID:12746456).