DAXX Polyklonaler Antikörper

DAXX Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (2)

Kat-Nr. : 20489-1-AP

Synonyme

ETS1 associated protein 1, EAP1, Death domain-associated protein 6, Death domain associated protein 6, DAP6

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  WB analysis of Y79 using 20489-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20489-1-AP (DAXX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20489-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20489-1-AP (DAXX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 20489-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20489-1-AP (DAXX antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

20489-1-AP bindet in WB, IHC, IF, ELISA DAXX und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DAXX fusion protein Ag14519
• Vollständiger Name: death-domain associated protein
• Berechnetes Molekulargewicht: 740 aa, 81 kDa
• Beobachtetes Molekulargewicht: 120 kDa, 70 kDa
• GenBank-Zugangsnummer: BC109074
• Gene symbol: DAXX
• Gene ID (NCBI): 1616
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Death associated protein 6 (DAXX) is a ubiquitous protein implicated in various cellular processes such as apoptosis, tumorigenesis, development and transcription. It has been shown to translocate from the nucleus to the cytoplasm under conditions of stress and to activate the Jun N-terminal kinase (JNK) pathway. Somatic mutations of death associated protein (DAXX) have been found in ALT cancers, including pancreatic neuroendocrine (panNET) cancers and glioblastoma multiforme (GBM) cancers.

Publikationen

Species	Application	Title
Mouse	WB	Cell Res; Suppression of MAPK11 or HIPK3 reduces mutant Huntingtin levels in Huntington's disease models.; Authors - Meng Yu
human	IF	Nat Commun; Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen.; Authors - Alice Mac Kain
mouse	WB	J Biol Chem; The histone chaperone Spt6 is required for activation-induced cytidine deaminase target determination through H3K4me3 regulation.; Authors - Nasim A Begum
		Sci Bull (Beijing); P53 upregulation by USP7-engaging molecular glues; Authors - Zhaoyang Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-13-2024)

The antibody strongly detected DAXX by western-blot (was used as a positive control in co-immunoprecipitation experiments) but also provided many other bands in HeLa cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

FH

Alexandros (Verified Customer) (03-03-2023)

Very happy with the final image we have in our blots compared to other antibodies we have previously used

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Adherent cells