DAXX Monoklonaler Antikörper

DAXX Monoklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

CloneNo.

1F3G9

Kat-Nr. : 67879-1-Ig

Synonyme

BING2, DAP6, DAXX, EAP1, ETS1 associated protein 1, hDaxx

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Galerie der Validierungsdaten

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  WB analysis of Raji using 67879-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 67879-1-Ig (DAXX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 67879-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 67879-1-Ig (DAXX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MOLT-4 using 67879-1-Ig

  MOLT-4 cells were subjected to SDS PAGE followed by western blot with 67879-1-Ig (DAXX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67879-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67879-1-Ig (DAXX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67879-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67879-1-Ig (DAXX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67879-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67879-1-Ig (DAXX antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67879-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67879-1-Ig (DAXX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 67879-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DAXX antibody (67879-1-Ig, Clone: 1F3G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, Daudi-Zellen, HeLa-Zellen, Jurkat-Zellen, MOLT-4-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67879-1-Ig bindet in WB, IHC, IF,ELISA DAXX und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DAXX fusion protein Ag14606
• Vollständiger Name: death-domain associated protein
• Berechnetes Molekulargewicht: 740 aa, 81 kDa
• Beobachtetes Molekulargewicht: 100-130 kDa
• GenBank-Zugangsnummer: BC109074
• Gene symbol: DAXX
• Gene ID (NCBI): 1616
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Death domain-associated protein (DAXX) is a transcription repressor involved in both physiological and pathological conditions. DAXX overexpression is a common feature in diverse cancers, which correlates with tumorigenesis, disease progression and treatment resistance. Daxx migrated with an apparent molecular weight of approximately 100-130 kDa. (PMID: 31350900, PMID: 32203224, PMID: 31614769)