DAZL Polyklonaler Antikörper

DAZL Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 12633-1-AP

Synonyme

DAZH, DAZL1, DAZLA, DAZ-like autosomal, Deleted in azoospermia-like

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  WB analysis using 12633-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12633-1-AP (DAZL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12633-1-AP

  IP result of anti-DAZL (IP:12633-1-AP, 4ug; Detection:12633-1-AP 1:500) with mouse testis tissue lysate 4400ug.

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  IHC staining of mouse testis using 12633-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12633-1-AP (DAZL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 12633-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12633-1-AP (DAZL antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 12633-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12633-1-AP (DAZL antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 12633-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12633-1-AP (DAZL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 12633-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12633-1-AP (DAZL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12633-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12633-1-AP (DAZL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 12633-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DAZL antibody (12633-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 12633-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DAZL antibody (12633-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 12633-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DAZL antibody (12633-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: Rattenhodengewebe, Maus-Eierstockgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 3 publications below

Produktinformation

12633-1-AP bindet in WB, IHC, IF-P, IP, ELISA DAZL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DAZL fusion protein Ag3326
• Vollständiger Name: deleted in azoospermia-like
• Berechnetes Molekulargewicht: 295 aa, 33 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC027595
• Gene symbol: DAZL
• Gene ID (NCBI): 1618
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DAZL proteins are germ cell-specific RNA-binding proteins and are considered major regulators of spermatogenesis. Because DAZL is a novel protein expressed specifically in germ cells, it is widely employed as a germ cell marker. In humans, immunostaining shows DAZL is abundant in the cytoplasm of primary spermatocytes, but scarce in spermatogonia.

Publikationen

Species	Application	Title
mouse	WB,IF	Environ Pollut; Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling; Authors - Yifan Hong
rat	IF	Stem Cell Res Ther; Concentrated exosomes from menstrual blood-derived stromal cells improves ovarian activity in a rat model of premature ovarian insufficiency.; Authors - Siwen Zhang
mouse,human	WB	Proteomics; Systematic in-depth proteomic analysis of mitochondria-associated endoplasmic reticulum membranes (MAM) in mouse and human testes.; Authors - Xiaoli Wang
mouse	WB	Front Endocrinol (Lausanne); Restoring Impaired Fertility Through Diet: Observations of Switching From High-Fat Diet During Puberty to Normal Diet in Adulthood Among Obese Male Mice.; Authors - Xiangyu Qi
chicken	IF	Front Genome Ed; Hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells; Authors - Yuzhou Gu