DAZL Polyklonaler Antikörper

DAZL Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 12633-1-AP

Synonyme

DAZ homolog, DAZ like autosomal, DAZH, DAZL, DAZL1, DAZLA, deleted in azoospermia like, Deleted in azoospermia like 1, SPGY like autosomal, SPGYLA

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Galerie der Validierungsdaten

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  WB analysis using 12633-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12633-1-AP (DAZL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12633-1-AP

  IP Result of anti-DAZL (IP:12633-1-AP, 4ug; Detection:12633-1-AP 1:500) with mouse testis tissue lysate 4400ug.

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  IHC staining of mouse testis using 12633-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12633-1-AP (DAZL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 12633-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12633-1-AP (DAZL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 12633-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12633-1-AP (DAZL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 12633-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12633-1-AP (DAZL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 12633-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DAZL antibody (12633-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse testis using 12633-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DAZL antibody (12633-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, Maus-Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 2 publications below

Produktinformation

12633-1-AP bindet in WB, IP, IHC, IF, ELISA DAZL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DAZL fusion protein Ag3326
• Vollständiger Name: deleted in azoospermia-like
• Berechnetes Molekulargewicht: 295 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC027595
• Gene symbol: DAZL
• Gene ID (NCBI): 1618
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF	Environ Pollut; Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling; Authors - Yifan Hong
rat	IF	Stem Cell Res Ther; Concentrated exosomes from menstrual blood-derived stromal cells improves ovarian activity in a rat model of premature ovarian insufficiency.; Authors - Siwen Zhang
human,mouse	WB	Proteomics; Systematic in-depth proteomic analysis of mitochondria-associated endoplasmic reticulum membranes (MAM) in mouse and human testes.; Authors - Xiaoli Wang
mouse	WB	Front Endocrinol (Lausanne); Restoring Impaired Fertility Through Diet: Observations of Switching From High-Fat Diet During Puberty to Normal Diet in Adulthood Among Obese Male Mice.; Authors - Xiangyu Qi