DAZL Rekombinanter Antikörper

DAZL Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251035E11

Kat-Nr. : 86344-3-PBS

Synonyme

DAZH, DAZL1, DAZLA, DAZ-like autosomal, Deleted in azoospermia-like

---

• 

  WB analysis using 86344-3-RR (same clone as 86344-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86344-3-RR (DAZL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86344-3-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 86344-3-RR (same clone as 86344-3-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 86344-3-RR (DAZL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86344-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 86344-3-RR (same clone as 86344-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86344-3-RR (DAZL antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86344-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 86344-3-RR (same clone as 86344-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86344-3-RR (DAZL antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86344-3-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 86344-3-RR (same clone as 86344-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DAZL antibody (86344-3-RR, Clone: 251035E11 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86344-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86344-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86344-3-RR against Human DAZL were performed. The affinity constant is 2.52 nM.

---

Geprüfte Anwendungen

Produktinformation

86344-3-PBS bindet in WB, IHC, IF-P, Indirect ELISA DAZL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DAZL fusion protein Ag37074
• Vollständiger Name: deleted in azoospermia-like
• Berechnetes Molekulargewicht: 295 aa, 33 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC027595
• Gene symbol: DAZL
• Gene ID (NCBI): 1618
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DAZL proteins are germ cell-specific RNA-binding proteins and are considered major regulators of spermatogenesis. Because DAZL is a novel protein expressed specifically in germ cells, it is widely employed as a germ cell marker. In humans, immunostaining shows DAZL is abundant in the cytoplasm of primary spermatocytes, but scarce in spermatogonia.