DAZL Rekombinanter Antikörper

DAZL Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

251035E11

Kat-Nr. : 86344-3-RR

Synonyme

DAZH, DAZL1, DAZLA, DAZ-like autosomal, Deleted in azoospermia-like

---

• 

  WB analysis using 86344-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86344-3-RR (DAZL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat testis using 86344-3-RR

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 86344-3-RR (DAZL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 86344-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86344-3-RR (DAZL antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 86344-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86344-3-RR (DAZL antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 86344-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DAZL antibody (86344-3-RR, Clone: 251035E11 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 86344-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86344-3-RR against Human DAZL were performed. The affinity constant is 2.52 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86344-3-RR bindet in WB, IHC, IF-P, ELISA DAZL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DAZL fusion protein Ag37074
• Vollständiger Name: deleted in azoospermia-like
• Berechnetes Molekulargewicht: 295 aa, 33 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC027595
• Gene symbol: DAZL
• Gene ID (NCBI): 1618
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DAZL proteins are germ cell-specific RNA-binding proteins and are considered major regulators of spermatogenesis. Because DAZL is a novel protein expressed specifically in germ cells, it is widely employed as a germ cell marker. In humans, immunostaining shows DAZL is abundant in the cytoplasm of primary spermatocytes, but scarce in spermatogonia.