DBR1 Monoklonaler Antikörper

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DBR1 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3B5A7

Kat-Nr. : 66951-1-PBS

Synonyme

DBR1, Lariat debranching enzyme

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  WB analysis of HeLa using 66951-1-Ig (same clone as 66951-1-PBS)

  WB result of DBR1 antibody (66951-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DBR1 transfected HeLa cells. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

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  WB analysis using 66951-1-Ig (same clone as 66951-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66951-1-Ig (same clone as 66951-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66951-1-Ig (same clone as 66951-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66951-1-Ig (same clone as 66951-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66951-1-Ig (same clone as 66951-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66951-1-Ig (same clone as 66951-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 66951-1-Ig (same clone as 66951-1-PBS)

  IP result of anti-DBR1 (IP:66951-1-Ig, 4ug; Detection:66951-1-Ig 1:2000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 66951-1-Ig (same clone as 66951-1-PBS)

  IP result of anti-DBR1 (IP:66951-1-Ig, 4ug; Detection:66951-1-Ig 1:2000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66951-1-Ig (same clone as 66951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66951-1-Ig (DBR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66951-1-Ig (same clone as 66951-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66951-1-Ig (DBR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66951-1-Ig (same clone as 66951-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DBR1 antibody (66951-1-Ig, Clone: 3B5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66951-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA DBR1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DBR1 fusion protein Ag8830
• Vollständiger Name: debranching enzyme homolog 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 544 aa, 62 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC009472
• Gene symbol: DBR1
• Gene ID (NCBI): 51163
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.