DCC Rekombinanter Antikörper

DCC Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250085A11

Kat-Nr. : 85787-4-PBS

Synonyme

Colorectal cancer suppressor, CRC18, CRCR1, IGDCC1

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  WB analysis of A-673 using 85787-4-RR (same clone as 85787-4-PBS)

  A-673 cells were subjected to SDS PAGE followed by western blot with 85787-4-RR (DCC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85787-4-PBS in a different storage buffer formulation.

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  WB analysis of U266 using 85787-4-RR (same clone as 85787-4-PBS)

  U266 cells were subjected to SDS PAGE followed by western blot with 85787-4-RR (DCC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85787-4-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP02122-3

  Sandwich ELISA standard curve of MP02122-3, Human DCC Recombinant Matched Antibody Pair - PBS only. 85787-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2937. 85787-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

• 

  Sample test of MP02122-3

  The mean DCC concentration was determined to be 11.21 ng/mL in SH-SY5Y cell extract based on a 1.00 mg/mL extract load and 210.63 ng/mL in mouse brain tissue extract based on a 6.60 mg/mL extract load.

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Geprüfte Anwendungen

Produktinformation

85787-4-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test DCC und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DCC fusion protein Eg2937
• Vollständiger Name: deleted in colorectal carcinoma
• Berechnetes Molekulargewicht: 158 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: NM_005215.3
• Gene symbol: DCC
• Gene ID (NCBI): 1630
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DCC, also named as IGDCC1, belongs to the immunoglobulin superfamily and DCC family. DCC is a receptor for netrin required for axon guidance. DCC mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. DCC gene is a tumor suppressor gene. This antibody raised against a synthesized polypeptide of human DCC protein recognizes endogenous DCC with an apparent molecular weight of 180 kDa. As the predicted molecular mass of DCC is 158 kDa, the slower mobility is likely attributable to post-translational modification, perhaps by glycosylation at potential N-linked glycosylation sites (PMID: 7926722; 8044801).