DCI Polyklonaler Antikörper

DCI Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11535-1-PBS

Synonyme

3,2-trans-enoyl-CoA isomerase, D3,D2-enoyl-CoA isomerase, Delta(3),Delta(2)-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, EC:5.3.3.8

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  WB analysis using 11535-1-AP (same clone as 11535-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  WB analysis using 11535-1-AP (same clone as 11535-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  WB analysis of human lung using 11535-1-AP (same clone as 11535-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11535-1-AP (same clone as 11535-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 11535-1-AP (same clone as 11535-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  WB analysis of BxPC-3 using 11535-1-AP (same clone as 11535-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11535-1-AP (same clone as 11535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11535-1-AP (DCI antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 11535-1-AP (same clone as 11535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11535-1-AP (DCI antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 11535-1-AP (same clone as 11535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11535-1-AP (DCI antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  IF Staining of BxPC-3 using 11535-1-AP (same clone as 11535-1-PBS)

  Immunofluorescent analysis of BxPC-3 cells using 11535-1-AP (DCI antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 11535-1-AP (same clone as 11535-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DCI antibody (11535-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11535-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA DCI und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DCI fusion protein Ag2094
• Vollständiger Name: dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase)
• Berechnetes Molekulargewicht: 302 aa, 33 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC020228
• Gene symbol: DCI
• Gene ID (NCBI): 1632
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ECI1(enoyl-CoA delta isomerase 1, mitochondrial) is also named as DCI(dodecenoyl-CoA isomerase) and belongs to the enoyl-CoA hydratase/isomerase family. It is able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. The full length protein has a transit peptide and 2 isoforms produced by alternative splicing and can exsit as a homotramer(PMID:15351645).