DCLK1 Polyklonaler Antikörper

DCLK1 Polyklonal Antikörper für WB, IHC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29800-1-PBS

Synonyme

DCAMKL1, DCDC3A, Doublecortin domain-containing protein 3A, Doublecortin-like and CAM kinase-like 1, Doublecortin-like kinase 1

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  WB analysis using 29800-1-AP (same clone as 29800-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29800-1-AP (DCLK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 29800-1-AP (same clone as 29800-1-PBS)

  IP result of anti-DCLK1 (IP:29800-1-AP, 4ug; Detection:29800-1-AP 1:50000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29800-1-AP (same clone as 29800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29800-1-AP (same clone as 29800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 29800-1-AP (same clone as 29800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 29800-1-AP (same clone as 29800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29800-1-AP (DCLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 29800-1-AP (same clone as 29800-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (29800-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 29800-1-AP (same clone as 29800-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DCLK1 antibody (29800-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29800-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29800-1-PBS bindet in WB, IHC, IF-P, IP, Indirect ELISA DCLK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DCLK1 fusion protein Ag32007
• Vollständiger Name: doublecortin-like kinase 1
• Berechnetes Molekulargewicht: 82KD
• Beobachtetes Molekulargewicht: 48 kDa, 82 kDa
• GenBank-Zugangsnummer: NM_004734
• Gene symbol: DCLK1
• Gene ID (NCBI): 9201
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.