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DCLK2 Polyklonaler Antikörper

DCLK2 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25450-1-PBS

Synonyme

DCAMKL2, CLICK-II, CLICK2, CLICK II, CL2

Filter:
  • Western Blot (WB) analysis of various lysates using DCLK2 Polyclonal antibody (25450-1-AP)

    WB analysis using 25450-1-AP (same clone as 25450-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25450-1-AP (DCLK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25450-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using DCLK2 Polyclonal antibody (25450-1-AP)

    IP experiment of mouse brain using 25450-1-AP (same clone as 25450-1-PBS)

    IP result of anti-DCLK2 (IP:25450-1-AP, 4ug; Detection:25450-1-AP 1:3000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 25450-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DCLK2 Polyclonal antibody (25450-1-AP)

    IF Staining of HepG2 using 25450-1-AP (same clone as 25450-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DCLK2 antibody (25450-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 25450-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

25450-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA DCLK2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen DCLK2 fusion protein Ag22142
Vollständiger Name doublecortin-like kinase 2
Berechnetes Molekulargewicht 766 aa, 84 kDa
Beobachtetes Molekulargewicht 83 kDa
GenBank-ZugangsnummerBC032726
Gene symbol DCLK2
Gene ID (NCBI) 166614
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DCLK2 belongs to the protein kinase superfamily, contains two N-terminal doublecortin domains, a C-terminal serine/threonine protein kinase domain, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains. DCLK2 is expressed in the brain, heart and eyes (PMID: 18075264).