- Featured Product
- KD/KO Validated
Decorin Polyklonaler Antikörper
Decorin Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte, zabrafish und mehr (1)
Anwendung
WB, IHC, IF/ICC, IF-P, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 14667-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | Mausherzgewebe, BxPC-3-Zellen, MCF-7-Zellen, Maus-Kolongewebe, NIH/3T3-Zellen, Rattenherzgewebe |
Erfolgreiche Detektion in IHC | humanes Hautkrebsgewebe, humanes Mammakarzinomgewebe, humanes Mammahyperplasie-Gewebe, humanes Kolonkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF-P | zebrafish retina, Mauslebergewebe |
Erfolgreiche Detektion in IF/ICC | BxPC-3-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunhistochemie (IHC) | IHC : 1:1000-1:4000 |
Immunfluoreszenz (IF)-P | IF-P : 1:50-1:500 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 2 publications below |
WB | See 21 publications below |
IHC | See 5 publications below |
IF | See 11 publications below |
Produktinformation
14667-1-AP bindet in WB, IHC, IF/ICC, IF-P, ELISA Decorin und zeigt Reaktivität mit human, Maus, Ratten, zabrafish
Getestete Reaktivität | human, Maus, Ratte, zabrafish |
In Publikationen genannte Reaktivität | human, hamster, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Decorin fusion protein Ag6328 |
Vollständiger Name | decorin |
Berechnetes Molekulargewicht | 359 aa, 40 kDa |
Beobachtetes Molekulargewicht | 70 kDa, 45-48 kDa |
GenBank-Zugangsnummer | BC005322 |
Gene symbol | Decorin |
Gene ID (NCBI) | 1634 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
---|---|
WB protocol for Decorin antibody 14667-1-AP | Protokoll herunterladen |
IHC protocol for Decorin antibody 14667-1-AP | Protokoll herunterladenl |
IF protocol for Decorin antibody 14667-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
---|---|
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Nat Commun The estrogen response in fibroblasts promotes ovarian metastases of gastric cancer | ||
Breast Cancer Res Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors | ||
Front Immunol Development of a prognostic model for hepatocellular carcinoma based on microvascular invasion characteristic genes by spatial transcriptomics sequencing | ||
PLoS Genet Impaired proteoglycan glycosylation, elevated TGF-β signaling, and abnormal osteoblast differentiation as the basis for bone fragility in a mouse model for gerodermia osteodysplastica. | ||
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kenzo (Verified Customer) (08-29-2023) | Antibody is working pretty well for IF on mouse tissue sections.
|
FH Huai-Chin (Verified Customer) (10-24-2022) | Good antibody with only single band. Actual band size is higher than the calculated protein size which may indicated some protein modification.
|
FH balawant (Verified Customer) (07-25-2022) | I have this antibody for my research it is working great in both tissue lysate as well as in cell lysate.
|
FH Iram (Verified Customer) (06-13-2022) | Excellent antibody for western blotting
|
FH Balawant (Verified Customer) (05-08-2022) | I have used this antibody. This antibody is excellent, and I used it for western blotting. The dilution of 1:1000 is functioning well.
|
FH Susmita (Verified Customer) (09-17-2020) | Antibody is working very good for WB
|