DCP1A Rekombinanter Antikörper

DCP1A Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242553F2

Kat-Nr. : 85047-5-RR

Synonyme

EC:3.6.1.62, HSA275986, mRNA decapping enzyme 1A, mRNA-decapping enzyme 1A, Nbla00360

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  WB analysis using 85047-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85047-5-RR (DCP1A antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 85047-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85047-5-RR (DCP1A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 85047-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85047-5-RR (DCP1A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 85047-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85047-5-RR (DCP1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 85047-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85047-5-RR (DCP1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 85047-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DCP1A antibody (85047-5-RR, Clone: 242553F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen, Maushirngewebe, Rattenhirngewebe, SW480-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85047-5-RR bindet in WB, IHC, IF/ICC, ELISA DCP1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DCP1A fusion protein Ag18027
• Vollständiger Name: DCP1 decapping enzyme homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 582 aa, 63 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC007439
• Gene symbol: DCP1A
• Gene ID (NCBI): 55802
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DCP1 decapping enzyme homolog A (S. cerevisiae), known as mRNA-decapping enzyme 1A (DCP1A),is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. This antibody specifically react with the 70kd DCP1A protein.