DCT Monoklonaler Antikörper

DCT Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B3D4

Kat-Nr. : 68114-1-PBS

Synonyme

1B3D4, DT, EC:5.3.3.12, L-dopachrome Delta-isomerase, L-dopachrome tautomerase

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  WB analysis of A375 using 68114-1-Ig (same clone as 68114-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68114-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 68114-1-Ig (same clone as 68114-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68114-1-PBS in a different storage buffer formulation.

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  WB analysis of pig skin using 68114-1-Ig (same clone as 68114-1-PBS)

  pig skin tissue were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68114-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 68114-1-Ig (same clone as 68114-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68114-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skin using 68114-1-Ig (same clone as 68114-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68114-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 68114-1-Ig (same clone as 68114-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using DCT antibody (68114-1-Ig, Clone: 1B3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68114-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68114-1-PBS bindet in WB, IF/ICC, Indirect ELISA DCT und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DCT fusion protein Ag26165
• Vollständiger Name: dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
• Berechnetes Molekulargewicht: 519 aa, 59 kDa
• Beobachtetes Molekulargewicht: 55-63 kDa
• GenBank-Zugangsnummer: BC028311
• Gene symbol: DCT
• Gene ID (NCBI): 1638
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).