DCTN1/p150 glued Polyklonaler Antikörper

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• KD/KO Validated

DCTN1/p150 glued Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 55182-1-AP

Synonyme

DCTN1, p150 glued, DP-150, DCTN 1, DAP-150

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  WB analysis of human brain using 55182-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55182-1-AP (DCTN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55182-1-AP

  WB result of DCTN1/p150 glued antibody (55182-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCTN1/p150 glued transfected HeLa cells.

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  WB analysis of Jurkat using 55182-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55182-1-AP (DCTN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 55182-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55182-1-AP (DCTN1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 55182-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55182-1-AP (DCTN1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MDCK using 55182-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 55182-1-AP (p150 glued antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

55182-1-AP bindet in WB, IHC, IF/ICC, ELISA DCTN1/p150 glued und zeigt Reaktivität mit human, Hund

• Getestete Reaktivität: human, Hund
• In Publikationen genannte Reaktivität: human, Miesmuschel
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: dynactin 1 (p150, glued homolog, Drosophila)
• Berechnetes Molekulargewicht: 142 kDa
• Beobachtetes Molekulargewicht: 135 kDa, 150 kDa
• GenBank-Zugangsnummer: NM_004082
• Gene symbol: p150 glued
• Gene ID (NCBI): 1639
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DCTN1, also named as DAP-150, p150-glued and p135, belongs to the dynactin 150 kDa subunit family. It is required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B). This antibody is specific to DCTN1.

Publikationen

Species	Application	Title
mussel	WB	Ecotoxicol Environ Saf; Proteomic profile in the mussel Perna viridis after short-term exposure to the brown tide alga Aureococcus anophagefferens.; Authors - Jian-Wei Zheng
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu
	IF	Clin Transl Med; Pathogenic variants of TUBB8 cause oocyte spindle defects by disrupting with EB1/CAKP5 interactions and potential treatment targeting microtubule acetylation through HDAC6 inhibition; Authors - Hui Luo