DCTPP1 Polyklonaler Antikörper

DCTPP1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 16684-1-AP

Synonyme

CDA03, dCTP pyrophosphatase 1, dCTPase 1, DCTPP1, Deoxycytidine triphosphatase 1, RS21C6, XTP3TPA

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  WB analysis of HeLa using 16684-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16684-1-AP (DCTPP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 16684-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16684-1-AP (DCTPP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 16684-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 16684-1-AP (DCTPP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 16684-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16684-1-AP (DCTPP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16684-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16684-1-AP (DCTPP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16684-1-AP

  Immunofluorescent analysis of HeLa cells using 16684-1-AP (DCTPP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mausherzgewebe, Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

16684-1-AP bindet in WB, IHC, IF/ICC, ELISA DCTPP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DCTPP1 fusion protein Ag10015
• Vollständiger Name: dCTP pyrophosphatase 1
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 19 kDa, 40 kDa, 80 kDa
• GenBank-Zugangsnummer: BC001344
• Gene symbol: DCTPP1
• Gene ID (NCBI): 79077
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DCTPP1, also named as XTP3TPA, CDAO3 and RS21C6, is a hydrolyzes deoxynucleoside triphosphates (dNTPs) to the corresponding nucleoside monophosphates. It has a central role in the balance of dCTP and the metabolism of deoxycytidine analogues, thus contributing to the preservation of genome integrity. DCTPP1 has some forms such as monomer (~20 kDa), Dimer (~40 kDa) and Homotetramer (~80 kDa).

Publikationen

Species	Application	Title
human	WB,IHC	J Cell Mol Med; Expression of human dCTP pyrophosphatase 1 (DCTPP1) and its association with cisplatin resistance characteristics in ovarian cancer; Authors - Yu Wang