DCUN1D1 Polyklonaler Antikörper

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• KD/KO Validated

DCUN1D1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 10965-1-AP

Synonyme

DCN1, DCN1 like protein 1, DCN1-like protein 1, DCNL1, DCUN 1D1

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  WB analysis using 10965-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10965-1-AP (DCUN1D1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10965-1-AP

  WB result of DCUN1D1 antibody (10965-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCUN1D1 transfected Jurkat cells.

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  IHC staining of human gliomas using 10965-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10965-1-AP (DCUN1D1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 10965-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10965-1-AP (DCUN1D1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

10965-1-AP bindet in WB, IHC, ELISA DCUN1D1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DCUN1D1 fusion protein Ag1440
• Vollständiger Name: DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC009478
• Gene symbol: DCUN1D1
• Gene ID (NCBI): 54165
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

It has been found that DCUN1D1, also known as squamous cell carcinoma-related oncogene, expression may play a role in development of brain metastasis in patients with Non-small cell lung carcinoma (NSCLC). DCUN1D1-positive tumor cells may have the ability to disrupt the blood-brain-barrier and colonize the brain. Those results suggest that DCUN1D1 may play a role in the brain parenchyma invasion.

Publikationen

Species	Application	Title
mouse,human	WB,IHC	Biochem Cell Biol; Evaluation of HZX-960, a novel DCN1-UBC12 interaction inhibitor, as a potential antifibrotic compound for liver fibrosis.; Authors - Wenjuan Zhou ; KD Validated
mouse	WB	Nature; A male germ-cell-specific ribosome controls male fertility; Authors - Huiling Li
human,mouse	WB,IHC	Clin Sci (Lond); Blockade of neddylation through targeted inhibition of DCN1 alleviates renal fibrosis; Authors - Jin-Ling Huo ; KD Validated