DCX Polyklonaler Antikörper

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• KD/KO Validated

DCX Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Konjugation

Unkonjugiert

Publikationen(46)

Rezensionen (3)

Kat-Nr. : 13925-1-AP

Synonyme

doublecortin, Doublin, Lissencephalin-X, Lis-X

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  WB analysis using 13925-1-AP

  Fetal human brain lysates were subjected to SDS PAGE followed by western blot with 13925-1-AP (DCX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13925-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13925-1-AP (DCX antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 13925-1-AP

  WB result of DCX antibody (13925-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCX transfected SH-SY5Y cells.

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  IHC staining of human gliomas using 13925-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13925-1-AP (DCX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 13925-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13925-1-AP (DCX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13925-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13925-1-AP (DCX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 13925-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using DCX antibody (13925-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 13925-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using DCX antibody (13925-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of SH-SY5Y using 13925-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DCX antibody (13925-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, fetales humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF-Fro: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 39 publications below

Produktinformation

13925-1-AP bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA DCX und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DCX fusion protein Ag4945
• Vollständiger Name: doublecortin
• Berechnetes Molekulargewicht: 402 aa, 45 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC027925
• Gene symbol: DCX
• Gene ID (NCBI): 1641
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.

Publikationen

Species	Application	Title
human	WB,IF	Nat Commun; m6a methylation orchestrates IMP1 regulation of microtubules during human neuronal differentiation; Authors - Pierre Klein
mouse	WB	Nat Commun; Ten-eleven translocation 1 mediated-DNA hydroxymethylation is required for myelination and remyelination in the mouse brain.; Authors - Ming Zhang
mouse	IF	Phytomedicine; Ginsenoside Rg1 enriches gut microbial indole-3-acetic acid to alleviate depression-like behavior in mice via oxytocin signaling; Authors - Siqi Yu
human	WB	PLoS Pathog; Human cytomegalovirus pUL97 upregulates SOCS3 expression via transcription factor RFX7 in neural progenitor cells; Authors - Xian-Zhang Wang
mouse	IF	Brain Behav Immun; Bacteroides species differentially modulate depression-like behavior via gut-brain metabolic signaling.; Authors - Youying Zhang
mouse	IF	J Neuroinflammation; Exosomes derived from bone-marrow mesenchymal stem cells alleviate cognitive decline in AD-like mice by improving BDNF-related neuropathology.; Authors - Sen Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sampath (Verified Customer) (12-02-2019)

It worked very well brain samples and gave me a strong signal for DCX at 1:1000 dilution for western blots. I had to increase the dilution to 1:2000. This is the antibody I was looking for. If you are looking for DCX antibody, I will recommend this.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000

FH

Sheed (Verified Customer) (01-26-2019)

Works well with Edu click reaction

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse

FH

Eleonora (Verified Customer) (12-11-2018)

The antibody works well, but it has too much interaction with the protein ladder (I have used Fisher BioReagent EZ-RUN).

• Applications: Western Blot,
• Primary Antibody Dilution: 1:600
• Cell Tissue Type: cortex (right) and hippocampus (left) mouse 3 weeks

DCX Antibody Western Blot, validation (1:600 dilution) in cortex (right) and hippocampus (left) mouse 3 weeks (Cat no:13925-1-AP)