DDA1 Polyklonaler Antikörper

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• KD/KO Validated

DDA1 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 14995-1-AP

Synonyme

C19orf58, DDA1, DET1 and DDB1 associated 1, PCIA 1, PCIA1

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Galerie der Validierungsdaten

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  WB analysis using 14995-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14995-1-AP (DDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14995-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14995-1-AP (DDA1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14995-1-AP

  IP result of anti-DDA1(IP:14995-1-AP, 4ug; Detection:14995-1-AP 1:500) with HepG2 cells lysate 1080 ug.

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  IF Staining of HepG2 using 14995-1-AP

  Immunofluorescent analysis of HepG2 cells using 14995-1-AP (DDA1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, Mauslebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

14995-1-AP bindet in WB, IP, IHC, IF, ELISA DDA1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDA1 fusion protein Ag6981
• Vollständiger Name: DET1 and DDB1 associated 1
• Berechnetes Molekulargewicht: 12 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC000615
• Gene symbol: DDA1
• Gene ID (NCBI): 79016
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDA1, also known as C19orf58 and PCIA1, belongs to the DDA1 family. DDA1 is an evolutionarily conserved gene located at 19p13.11 that may be involved in the activation of nuclear factor kappaB (NFκB). DDA1 encodes an 12 kDa protein with 102 AA residues whose orthologs share 82-92% identity with Arabidopsis, invertebrates, and vertebrates. Additionally, DDA1 might be involved in tumour formation and progression (PMID: 26942699, 28211159).

Publikationen

Species	Application	Title
human	WB	Nature; CRL4AMBRA1 is a master regulator of D-type cyclins.; Authors - Daniele Simoneschi
human	WB	Science; Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15.; Authors - Ting Han
human	WB	Nat Chem Biol; Structural basis of indisulam-mediated RBM39 recruitment to DCAF15 E3 ligase complex.; Authors - Dirksen E Bussiere ; KD Validated
mouse	IP, WB	Curr Biol; Cryptochromes-Mediated Inhibition of the CRL4Cop1-Complex Assembly Defines an Evolutionary Conserved Signaling Mechanism.; Authors - Luca Rizzini
human	IHC,IF	EMBO Mol Med; A clinically compatible drug-screening platform based on organotypic cultures identifies vulnerabilities to prevent and treat brain metastasis.; Authors - Lucía Zhu
human	WB	Cell Rep; Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15.; Authors - Tabitha C Ting