DDAH1 Monoklonaler Antikörper

DDAH1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A1A6

Kat-Nr. : 68823-1-Ig

Synonyme

DDAH, DDAH 1, DDAH1, DDAHI, Dimethylargininase 1, FLJ21264, FLJ25539

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  WB analysis using 68823-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68823-1-Ig (DDAH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68823-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68823-1-Ig (DDAH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Calu-3 cells, HT-29-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68823-1-Ig bindet in WB, ELISA DDAH1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DDAH1 fusion protein Ag32840
• Vollständiger Name: dimethylarginine dimethylaminohydrolase 1
• Berechnetes Molekulargewicht: 31kd
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: NM_012137
• Gene symbol: DDAH1
• Gene ID (NCBI): 23576
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dimethylarginine dimethylaminohydrolase 1 (DDAH1) is an enzyme that can degrade asymmetric dimethylarginine (ADMA), an endogenous nitric oxide synthase (NOS)inhibitor (PMID:26996393). About 80% of endogenous ADMA is metabolized by DDAH, principally the DDAH1 isoform (PMID:22460174). It was reported that DDAH1 is highly expressed in vascular endothelial cells in hearts (PMID:19917889). The observed molecular weight of DDAH1 is 35-40 kDa in the literature.