DDAH2 Polyklonaler Antikörper

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• KD/KO Validated

DDAH2 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14966-1-PBS

Synonyme

DDAH, DDAH 2, DDAH-2, DDAHII, Dimethylargininase 2

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  WB analysis using 14966-1-AP (same clone as 14966-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14966-1-AP (same clone as 14966-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 14966-1-AP (same clone as 14966-1-PBS)

  WB result of DDAH2 antibody (14966-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDAH2 transfected MCF-7 cells. This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14966-1-AP (same clone as 14966-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

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  IP experiment of COLO 320 using 14966-1-AP (same clone as 14966-1-PBS)

  IP result of anti-DDAH2 (IP:14966-1-AP, 3ug; Detection:14966-1-AP 1:500) with COLO 320 cells lysate 2000ug. This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 14966-1-AP (same clone as 14966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 14966-1-AP (same clone as 14966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 14966-1-AP (same clone as 14966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 14966-1-AP (same clone as 14966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14966-1-PBS bindet in WB, IHC, IP, Indirect ELISA DDAH2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDAH2 fusion protein Ag6831
• Vollständiger Name: dimethylarginine dimethylaminohydrolase 2
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC001435
• Gene symbol: DDAH2
• Gene ID (NCBI): 23564
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The DDAH family of proteins is formed by two isoforms, DDAH-1 and DDAH-2, both of which utilize ADMA as their substrate and increase NOS activity. DDAH-2,also named as DDAH, G6A, NG30, is the most prevalent isoform in blood vessels and endothelium and is important for embryonic development. It has also been linked to other diseases, such as hypertension, cerebral hemorrhage, cardiac disease, and diabetes. In cultured human endothelial cells,DDAH-2 had been found only in the cytosol, but in the nuclei of rat vascular smooth muscle cells.(PMID:21898353).