DDAH2 Monoklonaler Antikörper

DDAH2 Monoklonal Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

human, Kaninchen, Maus, Ratte

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A10G3

Kat-Nr. : 68154-1-PBS

Synonyme

Dimethylargininase 2, DDAHII, DDAH-2, DDAH 2, DDAH

---

• 

  WB analysis using 68154-1-Ig (same clone as 68154-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68154-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse retina using 68154-1-Ig (same clone as 68154-1-PBS)

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68154-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat colon using 68154-1-Ig (same clone as 68154-1-PBS)

  rat colon tissue were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68154-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50417-2

  Cytometric bead array standard curve of MP50417-2, DDAH2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68154-4-PBS. Detection antibody: 68154-1-PBS. Standard:Ag7127. Range: 0.391-50 ng/mL.

---

Geprüfte Anwendungen

Produktinformation

68154-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA DDAH2 und zeigt Reaktivität mit human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DDAH2 fusion protein Ag7127
• Vollständiger Name: dimethylarginine dimethylaminohydrolase 2
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC001435
• Gene symbol: DDAH2
• Gene ID (NCBI): 23564
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The DDAH family of proteins is formed by two isoforms, DDAH-1 and DDAH-2, both of which utilize ADMA as their substrate and increase NOS activity. DDAH-2,also named as DDAH, G6A, NG30, is the most prevalent isoform in blood vessels and endothelium and is important for embryonic development. It has also been linked to other diseases, such as hypertension, cerebral hemorrhage, cardiac disease, and diabetes. In cultured human endothelial cells,DDAH-2 had been found only in the cytosol, but in the nuclei of rat vascular smooth muscle cells.