• Featured Product
  • KD/KO Validated

DDB1 Polyklonaler Antikörper

DDB1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 11380-1-AP

Synonyme

UV DDB1, HBV X-associated protein 1, DNA damage-binding protein a, DNA damage-binding protein 1, DDBA



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHCT 116-Zellen, C2C12-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe, humanes Nierengewebe, humanes Plazenta-Gewebe, Jurkat-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen, Maushodengewebe, NIH/3T3-Zellen, Rattenhodengewebe
Erfolgreiche IPJurkat-Zellen
Erfolgreiche Detektion in IHChumanes Kolonkarzinomgewebe, human colon cancer
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:16000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

11380-1-AP bindet in WB, IHC, IF, IP, CoIP, ChIP, ELISA DDB1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen DDB1 fusion protein Ag1901
Vollständiger Name damage-specific DNA binding protein 1, 127kDa
Berechnetes Molekulargewicht 1140 aa, 127 kDa
Beobachtetes Molekulargewicht 127 kDa
GenBank-ZugangsnummerBC011686
Gene symbol DDB1
Gene ID (NCBI) 1642
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDB1, also named as XAP1, XPCe, DDBa and XPE-BF, belongs to the DDB1 family. It is required for DNA repair. DDB1 binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. This antibody is specific to DDB1.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for DDB1 antibody 11380-1-APProtokoll herunterladen
IHC protocol for DDB1 antibody 11380-1-APProtokoll herunterladenl
IP protocol for DDB1 antibody 11380-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Front Immunol

Ddb1 Is Essential for the Expansion of CD4+ Helper T Cells by Regulating Cell Cycle Progression and Cell Death.

Authors - Lingtao Yang
humanWB,IF

FASEB J

DDB2 promotes melanoma cell growth by transcriptionally regulating the expression of KMT2A and predicts a poor prognosis

Authors - Changlin Zhang
CoIP

iScience

A Multidimensional Characterization of E3 Ubiquitin Ligase and Substrate Interaction Network.

Authors - Di Chen
humanWB

PLoS One

14-3-3ε mediates the cell fate decision-making pathways in response of hepatocellular carcinoma to Bleomycin-induced DNA damage.

Authors - Tang Siwei S
humanWB

Virology

DDB1 is a cellular substrate of NS3/4A protease and required for hepatitis C virus replication.

Authors - Kang Xi X
  • KD Validated
humanWB

Cell Death Dis

Cul4 E3 ubiquitin ligase regulates ovarian cancer drug resistance by targeting the antiapoptotic protein BIRC3.

Authors - Xiaoyan Hu
  • KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Bernadette (Verified Customer) (09-24-2025)

The antibody worked well at 1:2000 dilution in 5% milk shaken overnight in 4 degree fridge on HEK293 cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HEK293T
FH

Sarah (Verified Customer) (07-03-2019)

Total cell lysate (15 ug) was resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk/0.1% Tween-20) for 1h. Membrane was incubated with anti-DDB1 in blocking buffer (1:1000) at 4C overnight. After washing, membrane was incubated in anti-rabbit-HRP in blocking bufffer (1:3000) for 1h at room temperature. Protein was detected using ECL reagent and imaged on a chemiluminescence detection system.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U251/Jurkat
DDB1 Antibody Western Blot, validation (1:1000 dilution) in U251/Jurkat (Cat no:11380-1-AP)