DDB1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

DDB1 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

4F6C6

Kat-Nr. : 66010-1-Ig

Synonyme

DDB p127 subunit, DDB1, DDBA, DNA damage binding protein 1, DNA damage binding protein a, HBV X associated protein 1, UV damaged DNA binding factor, UV DDB 1, UV DDB1, XAP 1, XAP1, XPCE, XPE, XPE BF, XPE binding factor

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• 

  WB analysis using 66010-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66010-1-Ig (DDB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of MCF-7 cells using 66010-1-Ig

  N/A incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66010-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66010-1-Ig (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 66010-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66010-1-Ig (DDB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human appendicitis using 66010-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66010-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66010-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66010-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 66010-1-Ig

  Immunofluorescent analysis of fixed MCF-7 cells using 66010-1-Ig (DDB1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, PC-12-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Appendizitis-Gewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 3 publications below

Produktinformation

66010-1-Ig bindet in WB, IHC, ELISA DDB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DDB1 fusion protein Ag17903
• Vollständiger Name: damage-specific DNA binding protein 1, 127kDa
• Berechnetes Molekulargewicht: 1140 aa, 127 kDa
• Beobachtetes Molekulargewicht: 127 kDa
• GenBank-Zugangsnummer: BC011686
• Gene symbol: DDB1
• Gene ID (NCBI): 1642
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDB1, also named as XAP1, XPCe, DDBa and XPE-BF, belongs to the DDB1 family. It is required for DNA repair. DDB1 binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. This antibody is specific to DDB1.

Publikationen

Species	Application	Title
human	WB	J Clin Invest; Cul4A-DDB1-mediated monoubiquitination of phosphoglycerate dehydrogenase promotes colorectal cancer metastasis via increased S-adenosylmethionine.; Authors - Yajuan Zhang ; KD Validated
human	WB	J Virol; Degradation of SAMHD1 by Vpx is Independent of Uncoating.; Authors - Paula Jáuregui ; KD Validated
human	WB	J Virol; Human herpesvirus 6A U4 inhibits proteasomal degradation of amyloid precursor protein.; Authors - Tian Tang ; KD Validated