DDHD2 Polyklonaler Antikörper

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• KD/KO Validated

DDHD2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 25203-1-AP

Synonyme

DDHD domain containing 2, DDHD2, KIAA0725, KIAA0725p, Phospholipase DDHD2, SAMWD1

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  WB analysis of rat brain using 25203-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25203-1-AP (DDHD2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 25203-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 25203-1-AP (DDHD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 25203-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25203-1-AP (DDHD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 25203-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25203-1-AP (DDHD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25203-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25203-1-AP (DDHD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 25203-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25203-1-AP (DDHD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 25203-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25203-1-AP (DDHD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 25203-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DDHD2 antibody (25203-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HEK-293 using 25203-1-AP

  Immunofluorescent analysis of HEK-293 cells using 25203-1-AP (DDHD2 antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, Mauslungengewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

25203-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA DDHD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDHD2 fusion protein Ag18373
• Vollständiger Name: DDHD domain containing 2
• Berechnetes Molekulargewicht: 711 aa, 81 kDa
• Beobachtetes Molekulargewicht: 80-90 kDa
• GenBank-Zugangsnummer: BC010504
• Gene symbol: DDHD2
• Gene ID (NCBI): 23259
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDHD2, also known as KIAA0725p, is a member of the intracellular phospholipase A1 (PLA1) protein family which comprises a group of enzymes that hydrolyze the sn-1 ester bond of phospholipids, producing 2-acyl-lysophospholipids and fatty acids. DDHD2 prefers phosphatidic acid as substrate and has a role in efficient membrane trafficking from the Golgi apparatus to the plasma membrane. The gene of DDHD2 maps to chromosome 8p11.23, and encodes a 711-amino-acid protein with a calculated molecular mass of 81 kDa. It has been reported that DDHD2 immunoblot analysis of various tissue extracts revealed two bands of 90 and 85 kDa (PMID: 11788596).

Publikationen

Species	Application	Title
human	WB	Protein Cell; Proteomic analysis of ferroptosis pathways reveals a role of CEPT1 in suppressing ferroptosis; Authors - Xiaoguang Liu
mouse		Neuron; Rewiring Neuronal Glycerolipid Metabolism Determines the Extent of Axon Regeneration.; Authors - Chao Yang ; KD Validated
mouse	WB	J Biol Chem; The phospholipase PNPLA7 functions as a lysophosphatidylcholine hydrolase and interacts with lipid droplets through its catalytic domain.; Authors - Christoph Heier
human	WB,IHC	Cell Death Dis; Circular RNA circRUNX1 promotes papillary thyroid cancer progression and metastasis by sponging MiR-296-3p and regulating DDHD2 expression.; Authors - Junjie Chu ; KD Validated
mouse	WB,IF	J Lipid Res; Cooperative lipolytic control of neuronal triacylglycerol by spastic paraplegia-associated enzyme DDHD2 and ATGL; Authors - Peter Hofer
rat	WB,IF,IP	EMBO J; The DDHD2-STXBP1 interaction mediates long-term memory via generation of saturated free fatty acids; Authors - Isaac O Akefe ; KO Validated