DDI1 Polyklonaler Antikörper

DDI1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 13968-1-AP

Synonyme

DDI1, Protein DDI1 homolog 1

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  WB analysis using 13968-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13968-1-AP (DDI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 13968-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13968-1-AP (DDI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13968-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13968-1-AP (DDI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Rattenhodengewebe, U-937-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

13968-1-AP bindet in WB, ELISA DDI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDI1 fusion protein Ag4992
• Vollständiger Name: DDI1, DNA-damage inducible 1, homolog 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 47 kDa, 56 kDa
• GenBank-Zugangsnummer: BC022017
• Gene symbol: DDI1
• Gene ID (NCBI): 414301
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ddi1(DNA-damage inducible 1)appears to release substrate from an ubiquitination complex, making the substrate available for deubiquitination to facilitate proteasomal degradation. Ddi1 could be a potential selective target for lung cancer treatment. Ddi1 runs as a doublet of 54/56 kDa on acrylamide gels besides the caculated MW 44 kDa.

Publikationen

Species	Application	Title
human	WB	J Mol Histol; Silibinin ameliorates cisplatin-induced acute kidney injury via activating Nfe2l1-mediated antioxidative response to suppress the ROS/MAPK signaling pathway.; Authors - Fang Yang
sheep	WB	Int J Mol Sci; The Effects of DDI1 on Inducing Differentiation in Ovine Preadipocytes via Oar-miR-432; Authors - Meilin Jin