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DDI2 Polyklonaler Antikörper

DDI2 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25377-1-PBS

Synonyme

Protein DDI1 homolog 2

Filter:
  • Western Blot (WB) analysis of various lysates using DDI2 Polyclonal antibody (25377-1-AP)

    WB analysis using 25377-1-AP (same clone as 25377-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25377-1-AP (DDI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25377-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DDI2 Polyclonal antibody (25377-1-AP)

    IF Staining of HepG2 using 25377-1-AP (same clone as 25377-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDI2 antibody (25377-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25377-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

25377-1-PBS bindet in WB, IF/ICC, Indirect ELISA DDI2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen DDI2 fusion protein Ag21953
Vollständiger Name DDI1, DNA-damage inducible 1, homolog 2 (S. cerevisiae)
Berechnetes Molekulargewicht 399 aa, 45 kDa
Beobachtetes Molekulargewicht 45-50 kDa
GenBank-ZugangsnummerBC006011
Gene symbol DDI2
Gene ID (NCBI) 84301
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDI2 contains 399 amino acids and contains an N-terminal ubiquitin-like (UBL) domain and a highly conserved retroviral protease-like (RVP) domain. DDI2 is an aspartic endoprotease that cleaves ubiquitylated target proteins to assist in their processing by the ubiquitin-proteasome system (UPS), especially when the UPS is inhibited(PMID: 32521225).