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CHOP/GADD153 Polyklonaler Antikörper

CHOP/GADD153 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (7)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(661)

Kat-Nr. : 15204-1-AP

Synonyme

CHOP; GADD153, CHOP, DDIT3, GADD153, CCAAT/enhancer-binding protein homologous protein



Geprüfte Anwendungen

Erfolgreiche Detektion in WBmit Tunicamycin behandelte HeLa-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen, RAW 264.7-Zellen
Erfolgreiche IPC6-Zellen
Erfolgreiche Detektion in IHChumanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Zervixkarzinomgewebe, humanes Schilddrüsenkarzinomgewebe, Maushirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCmit Tunicamycin behandelte HeLa-Zellen
Erfolgreiche Detektion in FC (Intra)mit Tunicamycin behandelte HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:3000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

15204-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA CHOP/GADD153 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, hamster, Hausschwein, Huhn, Hund, Kaninchen, Maus, Ratte, Rind, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CHOP/GADD153 fusion protein Ag7354
Vollständiger Name DNA-damage-inducible transcript 3
Berechnetes Molekulargewicht 19 kDa
Beobachtetes Molekulargewicht30 kDa
GenBank-ZugangsnummerBC003637
Gene symbol CHOP
Gene ID (NCBI) 1649
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CHOP/GADD153 antibody 15204-1-APProtokoll herunterladen
IHC protocol for CHOP/GADD153 antibody 15204-1-APProtokoll herunterladenl
IF protocol for CHOP/GADD153 antibody 15204-1-APProtokoll herunterladen
IP protocol for CHOP/GADD153 antibody 15204-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Nat Immunol

Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.

Authors - Li Jianhua J
humanWB

Mol Cell

Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis

Authors - Yi Fu
mouseIF

Acta Neuropathol

Wolframin is a novel regulator of tau pathology and neurodegeneration.

Authors - Shuo Chen
humanWB

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
humanWB,IHC,IP,ChIP

Mol Cell

The FUS::DDIT3 fusion oncoprotein inhibits BAF complex targeting and activity in myxoid liposarcoma.

Authors - Hayley J Zullow
humanWB

Cell Rep Med

Development of an orally bioavailable CDK12/13 degrader and induction of synthetic lethality with AKT pathway inhibition

Authors - Yu Chang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Morgane (Verified Customer) (09-15-2025)

Very specific, good signal, stress inductible

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HCT116
CHOP/GADD153 Antibody Western Blot validation (1/1000 dilution) in HCT116 (Cat no:15204-1-AP)
FH

Fan (Verified Customer) (03-22-2021)

Mouse retinal tissue was used. Single band detected at the right size.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
CHOP/GADD153 Antibody Western Blot validation (1:500 dilution) in (Cat no:15204-1-AP)
FH

Daniel (Verified Customer) (06-25-2019)

Specific staining detected in the proximal tubules.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse kidney (TRACK model)
CHOP/GADD153 Antibody Immunohistochemistry, validation (1:100 dilution) in Mouse kidney (TRACK model) (Cat no:15204-1-AP)