CHOP; GADD153 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CHOP; GADD153 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (5)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

459

Rezensionen (2)

Kat-Nr. : 15204-1-AP

Synonyme

C/EBP homologous protein, C/EBP homologous protein 10, CEBPZ, CHOP, CHOP 10, CHOP; GADD153, CHOP10, DDIT 3, DDIT3, GADD153

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 15204-1-AP

  WB result of CHOP antibody (15204-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHOP transfected HeLa cells.

• 

  WB analysis using 15204-1-AP

  Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15204-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15204-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15204-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15204-1-AP (CHOP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of C6 using 15204-1-AP

  IP result of anti-CHOP; GADD153(IP:15204-1-AP, 4ug; Detection:15204-1-AP 1:4000) with C6 cells lysate 1600 ug.

• 

  IHC staining of human colon cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15204-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15204-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using CHOP; GADD153 antibody (15204-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 15204-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using CHOP; GADD153 antibody (15204-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 15204-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CHOP; GADD153 (15204-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

• 

  FC experiment of HeLa using 15204-1-AP

  1X10^6 HeLa cells untreated (dashed lines) or treated with Tunicamycin (20 μg/mL, 4 h; red) were intracellularly stained with 0.4 ug Anti-Human CHOP; GADD153 (15204-1-AP) and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.4 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Tunicamycin behandelte HeLa-Zellen, C6-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche IP: C6-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Schilddrüsenkarzinomgewebe, humanes Zervixkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: mit Tunicamycin behandelte HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:500
• Immunfluoreszenz (IF): IF : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 21 publications below
• WB: See 422 publications below
• IHC: See 49 publications below
• IF: See 43 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

15204-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA CHOP; GADD153 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Hausschwein, Huhn, Maus, Ratte, Rind, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHOP; GADD153 fusion protein Ag7354
• Vollständiger Name: DNA-damage-inducible transcript 3
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC003637
• Gene symbol: DDIT3
• Gene ID (NCBI): 1649
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

Publikationen

Species	Application	Title
human	WB	Nat Immunol; Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.; Authors - Li Jianhua J
human	WB	Mol Cell; Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis; Authors - Yi Fu
mouse	IF	Acta Neuropathol; Wolframin is a novel regulator of tau pathology and neurodegeneration.; Authors - Shuo Chen
human	WB,IHC,IP,ChIP	Mol Cell; The FUS::DDIT3 fusion oncoprotein inhibits BAF complex targeting and activity in myxoid liposarcoma.; Authors - Hayley J Zullow
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
mouse,rat	WB	Cell Death Differ; Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation; Authors - Guangjin Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Fan (Verified Customer) (03-22-2021)

Mouse retinal tissue was used. Single band detected at the right size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500

CHOP; GADD153 Antibody Western Blot validation (1:500 dilution) in (Cat no:15204-1-AP)

FH

Daniel (Verified Customer) (06-25-2019)

Specific staining detected in the proximal tubules.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse kidney (TRACK model)

CHOP; GADD153 Antibody Immunohistochemistry, validation (1:100 dilution) in Mouse kidney (TRACK model) (Cat no:15204-1-AP)

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