DDOST Polyklonaler Antikörper

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DDOST Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Rezensionen (1)

Kat-Nr. : 14916-1-AP

Synonyme

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit, KIAA0115, OK/SW cl.45, OK/SW-cl.45, OST48

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  WB analysis using 14916-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14916-1-AP (DDOST antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14916-1-AP

  WB result of DDOST antibody (14916-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDOST transfected HeLa cells.

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  WB analysis of A549 using 14916-1-AP

  WB result of DDOST antibody (14916-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDOST transfected A549 cells.

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  IP experiment of mouse pancreas using 14916-1-AP

  IP result of anti-DDOST (IP:14916-1-AP, 4ug; Detection:14916-1-AP 1:1000) with mouse pancreas tissue lysate 3200 ug.

• 

  IHC staining of mouse testis using 14916-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14916-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 14916-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat small intestine using 14916-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse pancreas using 14916-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse pancreas using 14916-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, Maus-Pankreasgewebe, Ratten-Pankreasgewebe
• Erfolgreiche IP: Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, Maus-Pankreasgewebe, Ratten-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

14916-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA DDOST und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDOST fusion protein Ag6727
• Vollständiger Name: dolichyl-diphosphooligosaccharide-protein glycosyltransferase
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC002594
• Gene symbol: DDOST
• Gene ID (NCBI): 1650
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,CoIP	Cell Metab; Protein O-GlcNAcylation and hexokinase mitochondrial dissociation drive heart failure with preserved ejection fraction; Authors - Yuki Tatekoshi
mouse,human	WB,IHC,IF	Nat Commun; Smooth muscle NF90 deficiency ameliorates diabetic atherosclerotic calcification in male mice via FBXW7-AGER1-AGEs axis; Authors - Fei Xie
human	WB	Nat Chem Biol; Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.; Authors - Cecilia Lopez-Sambrooks
mouse	WB	J Agric Food Chem; Comparative Study of the Effects of Dietary-Free and -Bound Nε-Carboxymethyllysine on Gut Microbiota and Intestinal Barrier; Authors - Xiaojin Yuan
mouse	WB,CoIP	Res Sq; Hexokinase-1 mitochondrial dissociation and protein O-GlcNAcylation drive heart failure with preserved ejection fraction; Authors - Hossein Ardehali
human	IHC	IET Syst Biol; An immune-related multi-omics analysis of dolichyl-diphosphooligosaccharide protein glycosyltransferase in glioma: Prognostic value exploration and competitive endogenous RNA network identification; Authors - Jie Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nicole (Verified Customer) (04-28-2022)

80µl RIPA Hela cell extract: Nitrocellulose: no signal PVDF: weak signal, double band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela