DDRGK1 Polyklonaler Antikörper

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• KD/KO Validated

DDRGK1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21445-1-PBS

Synonyme

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  WB analysis using 21445-1-AP (same clone as 21445-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21445-1-AP (DDRGK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21445-1-AP (same clone as 21445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21445-1-AP (same clone as 21445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 21445-1-AP (same clone as 21445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 21445-1-AP (same clone as 21445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 21445-1-AP (same clone as 21445-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDRGK1 antibody (21445-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21445-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA DDRGK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDRGK1 fusion protein Ag13742
• Vollständiger Name: DDRGK domain containing 1
• Berechnetes Molekulargewicht: 314 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36-46 kDa
• GenBank-Zugangsnummer: BC000643
• Gene symbol: DDRGK1
• Gene ID (NCBI): 65992
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDRGK1, also name as C20orf116, is a member of the DDRGK domain-containing protein family with unknown function. Depletion of DDRGK1 inhibits cell proliferation and invasion. Expression of NF-κB target genes showed the most significant decrease after depleting of DDRGK1, suggesting that DDRGK1 may play an important role in the NF-κB signaling pathway. DDRGK1 interacts with IκBα and regulates its stability, thereby regulates the NF-κB transcriptional activity. DDRGK1 acts as an important regulator of the NF-κB pathway. (PMID: 23675531) Catalog#21445-1-AP is a rabbit polyclonal antibody raised against the full-length of human DDRGK1.